Mutant search tool (e-tilling)
query (input):
Database:
cutoff settings: GATK's GQ :    read depth :    lower %ALT :    upper %ALT :    num-ALT allowed in population :   

[Note] This mutant search tool is based on the exome-seq study presented in the following paper:
Yano and Hoshikawa et al. (2019) Plant Biotechnology "Multiplex exome sequencing reveals genome-wide frequency and distribution of mutations in the 'Micro-Tom' Targeting Induced Local Lesions in Genomes (TILLING) mutant library"

*This app is trial version and may be integragrated to other DB in future without notice.



[! This is example]


Summary | selected DB=[Micro-Tom 96-plex exome (variant dataset of 95 mutants using 10 WT controls for subtraction, reference=Heinz SL3.0)]
Query mutant
found
variant
found
aa
changing
nonsense Arabidopsis best homologue Arabidopsis homologue function E-value score
Solyc05g050010 6 6 3 2 => AT3G61510.1 (ACS1) ACC synthase 1 0 612
Solyc01g095080 7 7 6 - => AT1G01480.1 (ACS2) 1-amino-cyclopropane-1-carboxylate synthase 2 0 665
Solyc08g081540 2 2 - - => AT4G11280.1 (ACS6) 1-aminocyclopropane-1-carboxylic acid (acc) synthase 6 0 656



mutant search result

*variant with higher [GQ] may be confident
*hom-alt=[homozygous ALT], htz=[heterozygous], hom-ref=[homozygous REF]


Query=[Solyc05g050010] | mutant found=[6] | variant found=[6] | homologue of [AT3G61510.1 (ACS1), ACC synthase 1]
NBRP
mutant ID
DP GQ %ALT Predicted
genotype
Chr. Pos. REF
[RC]
ALT
[RC]
Transcript located at mutation type aa change alignment
CDS
alignment
Protein
TOMJPG1859_1 7 69 28.6 htz SL3.0ch05 60739859 G T Solyc05g050010.3.1 CDS.1 nonsense E60* [link] [link]
TOMJPW550_1 8 59 25.0 htz SL3.0ch05 60740039 C A Solyc05g050010.3.1 intron.1 intron or non-CDS - - -
TOMJPW558_1 5 40 60.0 htz SL3.0ch05 60740470 G T Solyc05g050010.3.1 intron.1 intron or non-CDS - - -
TOMJPW601_1 6 31 33.3 htz SL3.0ch05 60740590 G T Solyc05g050010.3.1 CDS.2 missense A145S [link] [link]
TOMJPE5405_5 7 28 28.6 htz SL3.0ch05 60740635 G T Solyc05g050010.3.1 CDS.2 nonsense G160* [link] [link]
TOMJPW1216_1 4 37 50.0 htz SL3.0ch05 60742397 G T Solyc05g050010.3.1 - exon (not CDS) - - -
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Query=[Solyc01g095080] | mutant found=[7] | variant found=[7] | homologue of [AT1G01480.1 (ACS2), 1-amino-cyclopropane-1-carboxylate synthase 2]
NBRP
mutant ID
DP GQ %ALT Predicted
genotype
Chr. Pos. REF
[RC]
ALT
[RC]
Transcript located at mutation type aa change alignment
CDS
alignment
Protein
TOMJPW339_1 4 37 50.0 htz SL3.0ch01 86329448 G [C] T [A] Solyc01g095080.3.1 CDS.4 missense P207Q [link] [link]
TOMJPW865_2 8 25 25.0 htz SL3.0ch01 86329461 T [A] C [G] Solyc01g095080.3.1 CDS.4 missense I203V [link] [link]
TOMJPW1065_1 11 66 36.4 htz SL3.0ch01 86329480 G [C] T [A] Solyc01g095080.3.1 CDS.4 missense N196K [link] [link]
TOMJPW927_1 8 25 25.0 htz SL3.0ch01 86329609 G A Solyc01g095080.3.1 intron.3 intron | within 2 bp from splicing junction (may affect) - - -
TOMJPW1065_1 8 64 25.0 htz SL3.0ch01 86330575 C [G] T [A] Solyc01g095080.3.1 CDS.3 missense G125D [link] [link]
TOMJPW840_1 8 25 25.0 htz SL3.0ch01 86330585 C [G] T [A] Solyc01g095080.3.1 CDS.3 missense V122I [link] [link]
TOMJPW518_1 4 37 50.0 htz SL3.0ch01 86331041 A [T] G [C] Solyc01g095080.3.1 CDS.1 missense W31R [link] [link]
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Query=[Solyc08g081540] | mutant found=[2] | variant found=[2] | homologue of [AT4G11280.1 (ACS6), 1-aminocyclopropane-1-carboxylic acid (acc) synthase 6]
NBRP
mutant ID
DP GQ %ALT Predicted
genotype
Chr. Pos. REF
[RC]
ALT
[RC]
Transcript located at mutation type aa change alignment
CDS
alignment
Protein
TOMJPE6034_1 7 28 28.6 htz SL3.0ch08 64689268 G T Solyc08g081540.3.1 intron.1 intron or non-CDS - - -
TOMJPW885_1 7 28 28.6 htz SL3.0ch08 64689773 T C Solyc08g081540.3.1 intron.3 intron or non-CDS - - -
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